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hom [2019-05-02 18:07:46]
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hom [2019-05-02 18:07:46]

homoserine dehydrogenase (NADPH)
Locus
BSU32260
Isoelectric point
4.90
Molecular weight
47.33 kDa
Protein length
433 aa Sequence Blast
Gene length
1302 bp Sequence Blast
Function
biosynthesis of methionine and threonine
Product
homoserine dehydrogenase (NADPH)
Essential
no
E.C.
1.1.1.3
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of methionine/ S-adenosylmethionine
  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of lysine/ threonine

    • Gene

    Coordinates
    3,314,828 3,316,129

    The protein

    Catalyzed reaction/ biological activity

  • L-aspartate 4-semialdehyde + NADPH+H+ --> L-homoserine + NADP+ PubMed

    • Protein family

  • Homoserine dehydrogenase family (single member, according to UniProt)

    • Domains

  • ACT domain (aa 350 ... 426) (according to the Interpro database)

    • Cofactors

  • NADPH PubMed

    • Structure

  • 2EJW (from Thermus thermophilus hb8, 37% identity, 57% similarity)

    • Localization

  • membrane associated PubMed

    • Additional information

  • belongs to the 100 most abundant proteins PubMed

    • Expression and Regulation

    Operons

    Genes
    yutH-hom-thrC-thrB
    Description
    PubMed

    Regulation

  • expressed during sporulation PubMed
    • view in new tab

    Genes
    hom-thrC-thrB
    Description
    PubMed

    Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon
  • TnrA: repression, PubMed, in TnrA regulon
  • ThrR: repression, PubMed, in ThrR regulon

    • Regulation

  • expressed in the presence of lysine or cysteine (ThrR) PubMed
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    Biological materials

    Mutant

  • BKE32260 (hom::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAAAAAACTCCACCTTTCT, downstream forward: _UP4_GTAGAAGGGAACGGTTGGAG
  • BKK32260 (hom::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAAAAAACTCCACCTTTCT, downstream forward: _UP4_GTAGAAGGGAACGGTTGGAG

    • Expression vector

  • pGP2297 (expression with SUMO protein and N-terminal His-tag from E. coli, in pET-SUMOadapt), available in Jörg Stülke's lab
  • pGP2300 (expression with N-terminal His-tag from E. coli, in pWH844), available in Jörg Stülke's lab

    • LacZ fusion

  • pBP312 (in pAC5), available in Fabian Commichau's lab PubMed

    • References

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